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lplD [2018-07-19 18:37:52]

?-galacturonidase

locus

BSU07130

5.36

49.32 kDa

protein length

446 aa Sequence Blast

gene length

1338 bp Sequence Blast

function

utilization of pectin

product

α-galacturonidase

essential

synonyms

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.26|Utilization of pectin]

[category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

Gene

Coordinates

782,958 → 784,298

The protein

Catalyzed reaction/ biological activity

cleavage of α-1,4-di-galacturonate [Pubmed|23416295]

Protein family

glycosyl hydrolase 4 family [Pubmed|23416295]

[SW|Domains]

active site motif: CHEV [Pubmed|23416295]

[SW|Cofactors]

NAD, Mn(2+) [Pubmed|23416295]

Structure

[PDB|3FEF]

Expression and Regulation

Operons

genes

[gene|18B09A645AC8E13A1AD3B73A0C828E5A0F5D42BB|lplD]-[gene|EBD0F966FD7B3662814D530064BD307561A5CB15|yetF]

description

[pubmed|22383849]

sigma factors

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Biological materials

Mutant

BKE07130 (Δ[gene|18B09A645AC8E13A1AD3B73A0C828E5A0F5D42BB|lplD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATATGAAACACTCCCT, downstream forward: _UP4_TGAGCTTGCCGATTAGCTCT

BKK07130 (Δ[gene|18B09A645AC8E13A1AD3B73A0C828E5A0F5D42BB|lplD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATATGAAACACTCCCT, downstream forward: _UP4_TGAGCTTGCCGATTAGCTCT

References

23416295